Component |
Version |
Inputs |
Outputs |
Description |
AffyReader |
1.1.2 |
affy, sampleNames |
expr, exprRaw, groups |
Imports gene expression or exon array data from Affymetrix CEL-files. |
AlignStats |
1.2 |
alignment, reference, annotation, refgene, chromsize, log |
report, stats |
Evaluate sequencing data, especially RNA-seq data quality using RSeQC. |
AutoGating |
1.0 |
rule, clusters, array |
filtered, filteredArray, population, populationArray |
Automatically gate cell populations. |
Blast |
1.0 |
query, dbSeq |
result |
Basic local alignment search tool. |
ClusterChooser |
1.0 |
population, plots, sampleAnnotation |
corrected, fileStatus |
Graphical tool for cluster population selection. |
ClusterFiltering |
1.0 |
fcsDir, in |
filtered, out |
Filters columns and/or rows from by clusters using flexible criteria. |
DEE2DEG |
1.0 |
dee, dbConnect |
deg |
Find differentially expressed genes (DEG) based on differentially expressed exons (DEE). |
EnsemblDNA |
1.0 |
regions, connection |
sequences |
Fetches DNA sequences from the Ensembl database. |
EnsemblGenes |
1.0 |
connection |
codingGene, otherGene |
Retrieves the Ensembl gene locations. |
ExonAnnotator |
1.0 |
query, dbConnect |
table |
Maps gene, transcript or exon
Ensembl IDs to one another via the Ensembl query interface. |
ExonExpression |
1.0 |
expr, sample, exon |
ExonExpr |
Converts exon expression matrix for a list of exons of interest. |
ExonToPeptide |
1.0 |
ExonList, connection |
ExonSeq, PeptideSeq |
Fetches exon-peptide sequences of query exon list. |
FASTA2CSV |
1.0 |
in |
out |
Converts a FASTA file to CSV. |
FastQC |
2.0 |
read, mate |
summary, report, extra, folder |
Short reads quality control for high-throughput sequencing data. |
GatingSummary |
1.0 |
summaries, sampleAnnotation, panel, population |
summaryResult, report |
Generate summay report table and heatmap. |
GetUtrSeq |
1.0 |
transcripts, connection |
UTR5, UTR3 |
Fetch UTR sequences of query transcripts. |
GlobalCorrelation |
1.2 |
gene, mirna |
correlated_filtered, correlated_all, expression_miRNA, expression_mRNA |
Compute correlation between miRNA and gene/transcript expression. |
GOClustering |
1.1.3 |
goAnnotations, expr, similarityTable |
report, clusters |
Clusters genes based on their gene ontology (GO) annotations. |
LimmaStat |
1.0 |
expr, sampleAnnotation |
sigTarget |
Select differentially expressed probesets, exons or transcripts
from probeset, exon or transcript expression data by using
limma statistical test. |
MeapNormalization |
2.0 |
affy, sampleNames |
normalized |
Prepares normalized data at probe level. |
MeapQuantification |
2.0 |
normalized, dbConnect |
ExonExpr, SpliceVariantExpr, GeneExpr |
Quantify expression data on exon, splicing variant or gene level. |
MeapVisualizer |
1.0 |
expr, geneList, dbConnect, sample |
report |
Generate visualization for genes of interest with spliced isoforms. |
MirMatch |
1.0 |
utrSeeds, matureSeq, pairs |
matched |
String match for between mature miRNA seeds and target UTR seeds. |
QCParser |
1.0 |
summary |
basicStats, perBaseSeqQual, perSeqQualScore, perBaseSeqContent, perBaseGC, perSeqGC, perBaseN, seqDuplicateLevel, overrepresented |
Parse SeqQC summary result. |
Trimmer |
1.0 |
data, perBaseSeqQual, trimInfo |
trimmedReads |
Trim sequence reads at both ends. |
Trimmomatic |
2.1 |
read, mate, adapter |
trimmedReads, stdout, stats, log |
Trimmomatic performs a variety of useful trimming tasks for
illumina paired-end and single ended data. |
UtrSeq2Seeds |
1.0 |
utrseq |
seeds |
Generate seeds with 15bp (XXXXXXXQXXXXXXX) with each query loci in middle. |