@HD	VN:1.0	SO:coordinate
@SQ	SN:test_chromosome	LN:650
@PG	ID:TopHat	VN:1.3.1	CL:/usr/local/bin/tophat -r 20 test_ref reads_1.fq reads_2.fq
test_mRNA_33_189_4a	89	test_chromosome	165	255	75M	*	0	0	TTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACCTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGGGCT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
test_mRNA_46_195_17	137	test_chromosome	96	255	75M	*	0	0	GTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	NH:i:1
test_mRNA_75_235_21	73	test_chromosome	125	255	75M	*	0	0	ACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGCACGATCGGACTGAGGAGGGCAGTAGAACGT	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:3	NH:i:1
test_mRNA_85_275_38	153	test_chromosome	351	255	50M100N25M	*	0	0	ACTCTTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTACTACTTGAGACTGGGATCGAG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:2	XS:A:+	NH:i:1
test_mRNA_94_291_40	153	test_chromosome	367	255	34M100N41M	*	0	0	CTTCCTGGGACGTAGACGGATCGGCAACGCGACATTTTCTACTTGAGACTGGGATCGAGGCGGACTTTTTGGGAC	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:5	XS:A:+	NH:i:1
test_mRNA_104_274_1c	89	test_chromosome	250	255	1M100N50M100N24M	*	0	0	CACTATTACTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGGGATCGA	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:0	XS:A:+	NH:i:1
test_mRNA_111_268_d	89	test_chromosome	244	255	7M100N50M100N18M	*	0	0	ATACGCCACTATTATTTTATTATCTTACTCGGACGTAGACGGATCGGCAACGGGACTTTTTCTACTTGAGACTGG	IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	XS:A:+	NH:i:1